Measurement

Part:BBa_K741004:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)

plac-RBS-cI-T-pRM-RBS-GFP-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1898


Design Notes

Protein CI is expressed to activated the promoter pRM. Thus different concentration of CI lead to different strength of green fluorescence. We use this part to test the character of modified pRM (BBa_I12007) being activated by CI.

Source

BBa_R0010 BBa_P0451 BBa_I13504 BBa_I12007

References